Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122500_7yjr TGCTTCCCAAGGGCCTCATGATCCAAGGAGTG(+CAGTGT)GTACCCACTTCTGCTGTAAAAGTCGTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605122458_p76i CTTCTTTATTAAATGAGCACACAGTATGCCTG(+CAGTGT)GTACCACATGAATTCTGTTTGTTCTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605122456_m2fj GCTAAAGAAAGAGAACAAACAGAATTCATGTG(+CAGTGT)GTACCAGGCATACTGTGTGCTCATTTAATAA NGG 10 8 17 8 24 0 100 5 10
    240605122455_bjtq CCTGGTGCCCGAGGAAGCCAGAGAGAATGTTG(+CAGTGT)GATCCCTGGAACTGGAATTAACAGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605122453_dqdq GCTCACAACTATCTGTTAATTCCAGTTCCAGG(+CAGTGT)GATCCAACATTCTCTCTGGCTTCCTCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605122451_6mhp CGTGGCTAGGAAGGGCCTGTCTTCCCAGCAGG(+CAGTGT)GATCCTCGCGGTCACAGGCTCCAGCCCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605122450_osfh CATTGATGGGGGCTGGAGCCTGTGACCGCGAG(+CAGTGT)GATCCCTGCTGGGAAGACAGGCCCTTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240605122448_sqje CCAGTGTGGTCGAGGGCCCTGGAGGCACTGGG(+CAGTGT)GTACCTCTGATTTTACATTTCTATCTTAATT NGG 10 8 17 8 24 0 100 5 10
    240605122447_bhg4 TGAATAATTAAGATAGAAATGTAAAATCAGAG(+CAGTGT)GTACCCCAGTGCCTCCAGGGCCCTCGACCAC NGG 10 8 17 8 24 0 100 5 10
    240605122445_ksj1 ATGCATGTTTAGTCACAGCAGTGAAGCAATGG(+CAGTGT)GTACCAGATGTTAAATGCTGGAACATTCCAA NGG 10 8 17 8 24 0 100 5 10