Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122557_ed1f AATGACATGAACTTATTAAGAATTAACTTAAG(+CAGTGT)GATCCAATATATATATATATATATAATATGT NGG 10 8 17 8 24 0 100 5 10
    240605122556_2f35 ATATAACATATTATATATATATATATATATTG(+CAGTGT)GATCCTTAAGTTAATTCTTAATAAGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240605122555_rcxt TGCTTAATTCTTTTAGCGAATGCACAGGTTAG(+CAGTGT)GTACCACCGTGAGGAAAAGGAGGGTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605122553_i7nf TCATTCCCCACACCCTCCTTTTCCTCACGGTG(+CAGTGT)GTACCTAACCTGTGCATTCGCTAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240605122552_ptwx TGCCTTCATCTCAGAATTGCTCATCCCAGGTG(+CAGTGT)GATCCCATGTGTCTGCAGTAGACCAGTCACC NGG 10 8 17 8 24 0 100 5 10
    240605122550_y0p7 ACAAAGGTGACTGGTCTACTGCAGACACATGG(+CAGTGT)GATCCACCTGGGATGAGCAATTCTGAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605122549_rf46 CACTTTGTAAGGAAAGTGGATACTTTAGAATG(+CAGTGT)GTACCCTATGCATCCAAATGAAATGTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605122548_vz5c ATGTAAAGGCACATTTCATTTGGATGCATAGG(+CAGTGT)GTACCATTCTAAAGTATCCACTTTCCTTACA NGG 10 8 17 8 24 0 100 5 10
    240605122546_8y2v AGACTAAGCAATAATATTTAGCTCTGGAATGG(+CAGTGT)GTACCATCTCCTTTTTCCTCATTAAAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605122545_v71o TGGTTGAGCCTTTAATGAGGAAAAAGGAGATG(+CAGTGT)GTACCCATTCCAGAGCTAAATATTATTGCTT NGG 10 8 17 8 24 0 100 5 10