Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122629_b4bf TATGCTACTTGCACTGTGACATGGCATTTGAG(+CAGTGT)GATCCCTGACTTACAGACAAAGCTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605122627_35jp TGTGTCATTTTAAGCTTTGTCTGTAAGTCAGG(+CAGTGT)GATCCTCAAATGCCATGTCACAGTGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605122626_51hm AAAGCTCATCACAAGGTCTACTAAGCATTAAG(+CAGTGT)GATCCTCATTATAAGTCCTCTGTACCACTTT NGG 10 8 17 8 24 0 100 5 10
    240605122624_vqte AAAAAAAAGTGGTACAGAGGACTTATAATGAG(+CAGTGT)GATCCTTAATGCTTAGTAGACCTTGTGATGA NGG 10 8 17 8 24 0 100 5 10
    240605122623_0dil AATACTGACTCTCATTAGATATGATCTTATAG(+CAGTGT)GATCCATACCCTCCCTAGGACCAAATATATC NGG 10 8 17 8 24 0 100 5 10
    240605122622_jciv CATCAGATATATTTGGTCCTAGGGAGGGTATG(+CAGTGT)GATCCTATAAGATCATATCTAATGAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605122619_4u84 GGCATGGCTCCTCGCCGCAGCTGTCTCCCTTG(+CAGTGT)GTACCGACCTCTCTGTGGGCTGGAGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605122618_qp7u CTTAGGCCTCTCTCCAGCCCACAGAGAGGTCG(+CAGTGT)GTACCAAGGGAGACAGCTGCGGCGAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605122617_1ldv CCTTGTCAAACCAAGTTGTCATGGATGCTTTG(+CAGTGT)GTACCCCTCCCCTCAGGGGCTTTGGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240605122615_9q9t TTCTTGTAATACCAAAGCCCCTGAGGGGAGGG(+CAGTGT)GTACCAAAGCATCCATGACAACTTGGTTTGA NGG 10 8 17 8 24 0 100 5 10