Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122730_5msc AATCTTTTCAGAGAATTCTAAAAAGACTATAG(+CAGTGT)GATCCTATAATAGATCCAATGATCTATAGTG NGG 10 8 17 8 24 0 100 5 10
    240605122728_g5cj TATGACACTATAGATCATTGGATCTATTATAG(+CAGTGT)GATCCTATAGTCTTTTTAGAATTCTCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605122727_xwtf GCTCACAACTGTTCGTAACTCAAGTTTCAGAG(+CAGTGT)GATCCAGTGCCCTCTTTGGACTCCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605122726_mw8b CTGGTGCCTGTGGGAGTCCAAAGAGGGCACTG(+CAGTGT)GATCCTCTGAAACTTGAGTTACGAACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605122725_tsmp TTGAGTTGAACTAGTGTTGAGGCTAGGCTTAG(+CAGTGT)GTACCATAGAACTTTGGTTTTTCAAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240605122724_borm GAAACCCTGTCTTGAAAAACCAAAGTTCTATG(+CAGTGT)GTACCTAAGCCTAGCCTCAACACTAGTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605122722_p0yl CTGACTGGCTCTGGCCTATGAACTCATTGCTG(+CAGTGT)GATCCATTAACCCTTTCCTAGCTTCAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605122721_6ueg GAATTACCTCTGAAGCTAGGAAAGGGTTAATG(+CAGTGT)GATCCAGCAATGAGTTCATAGGCCAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605122719_9nq7 ACTTTCTCTGAGCTGCTTTTATGCATATTGGG(+CAGTGT)GTACCTAGTAGTCTTCCCAACCTTTAACACT NGG 10 8 17 8 24 0 100 5 10
    240605122718_olgj CTCACAGTGTTAAAGGTTGGGAAGACTACTAG(+CAGTGT)GTACCCCAATATGCATAAAAGCAGCTCAGAG NGG 10 8 17 8 24 0 100 5 10