Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122759_0810 GTTACAGATTGCTCTTTATTATAATACCAGTG(+CAGTGT)GTACCCAAAGAAAAAAGCAGAGCATTATGTA NGG 10 8 17 8 24 0 100 5 10
    240605122757_hnz5 AAACTTACATAATGCTCTGCTTTTTTCTTTGG(+CAGTGT)GTACCACTGGTATTATAATAAAGAGCAATCT NGG 10 8 17 8 24 0 100 5 10
    240605122755_ahrg TCACATCACTGGTGTGAAGTGATGTGAAAAGG(+CAGTGT)GTACCTTCATCGAGTGCTGTACCTGCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605122754_cww6 GCACAGAACAGCAGGTACAGCACTCGATGAAG(+CAGTGT)GTACCCTTTTCACATCACTTCACACCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605122753_92ev GCATCACAATTATTCATTTGTGGAACATTTTG(+CAGTGT)GTACCTGAAATATTTACATAAGAAGGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240605122752_f2cu TGGGTTTCCGCCTTCTTATGTAAATATTTCAG(+CAGTGT)GTACCAAAATGTTCCACAAATGAATAATTGT NGG 10 8 17 8 24 0 100 5 10
    240605122750_wgl2 TCGCTTGGAGGGTGGACAAGGGGCAGTCCTAG(+CAGTGT)GTACCAGGCTCTCAGTCTCGAGCATCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605122748_l1k6 CGGTGTCTGGGATGCTCGAGACTGAGAGCCTG(+CAGTGT)GTACCTAGGACTGCCCCTTGTCCACCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605122747_5yxy CAGACCTGGAAGTCCTCTAGTGTATGTGTTAG(+CAGTGT)GATCCTCATATCAGCTGATGTATGCTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605122746_9gbr CCAAACAAACAGCATACATCAGCTGATATGAG(+CAGTGT)GATCCTAACACATACACTAGAGGACTTCCAG NGG 10 8 17 8 24 0 100 5 10