Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122828_cmgg TGAGTGTCTGTTCTGTCCTGTTGGACCTCATG(+CAGTGT)GTACCCACACTTGAGGCAGCTTGTTTCCTTA NGG 10 8 17 8 24 0 100 5 10
    240605122826_obj9 CTGGGTAAGGAAACAAGCTGCCTCAAGTGTGG(+CAGTGT)GTACCATGAGGTCCAACAGGACAGAACAGAC NGG 10 8 17 8 24 0 100 5 10
    240605122825_84al TTGTTAAAGGATTTGAGGGTTTTTCTCTGCAG(+CAGTGT)GTACCACCTGTAAATAAGAAGAAAGTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605122823_p8ke TTTGGCCCCTACTTTCTTCTTATTTACAGGTG(+CAGTGT)GTACCTGCAGAGAAAAACCCTCAAATCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605122822_8klc ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+CAGTGT)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605122820_ai29 CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+CAGTGT)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605122819_wdyv TACCTTTAATCTTAGCACTTGAGAGGCAGAGG(+CAGTGT)GATCCCCGAGTTAGAGATCAGCCTTGTTTAT NGG 10 8 17 8 24 0 100 5 10
    240605122817_zgbm TTGAAATAAACAAGGCTGATCTCTAACTCGGG(+CAGTGT)GATCCCTCTGCCTCTCAAGTGCTAAGATTAA NGG 10 8 17 8 24 0 100 5 10
    240605122815_fupt ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+CAGTGT)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605122814_jetl CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+CAGTGT)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10