Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122840_z9fp TCACTTCAATCTCTTCGTCACATATTGGGGTG(+CAGTGT)GTACCAAGGAAGGTGACCAGTATTGTTTACA NGG 10 8 17 8 24 0 100 5 10
    240605122839_arx0 TCATCTGTAAACAATACTGGTCACCTTCCTTG(+CAGTGT)GTACCACCCCAATATGTGACGAAGAGATTGA NGG 10 8 17 8 24 0 100 5 10
    240605122837_ll31 CATGCTGGCAACATCTGATTAAAGGCAGTGAG(+CAGTGT)GATCCAAGACGGATGGAGTGTCTTAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605122836_navs GATGTACACACTAAGACACTCCATCCGTCTTG(+CAGTGT)GATCCTCACTGCCTTTAATCAGATGTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605122835_m0ih AGTGATCGAGGGGGGAGGGCCCCTTGTGCATG(+CAGTGT)GTACCATCTCTAGGCTGGTAGTCTTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605122834_56rb TTATAGAACTCAAGACTACCAGCCTAGAGATG(+CAGTGT)GTACCATGCACAAGGGGCCCTCCCCCCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605122833_s2dd AGCCCTTGGAGGAGTCTAGAAGATGGTGAGTG(+CAGTGT)GATCCCAGACATTGGACCGGTGGAGTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605122831_6ft7 GCAAAATCAAACTCCACCGGTCCAATGTCTGG(+CAGTGT)GATCCACTCACCATCTTCTAGACTCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605122830_d638 AAAAAATGAGACTTTGACAATTAGTTGGAAAG(+CAGTGT)GATCCAACTGAAATTGTGGTACATTTTACTT NGG 10 8 17 8 24 0 100 5 10
    240605122829_ea6l TGTGTAAGTAAAATGTACCACAATTTCAGTTG(+CAGTGT)GATCCTTTCCAACTAATTGTCAAAGTCTCAT NGG 10 8 17 8 24 0 100 5 10