Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122943_7flc CCAGACAAATGTAGTTTACTGCAGGCTTACTG(+CAGTGT)GATCCTGACATTTAATTAAATATAACATAAT NGG 10 8 17 8 24 0 100 5 10
    240605122942_6hp4 ACTAGATTATGTTATATTTAATTAAATGTCAG(+CAGTGT)GATCCAGTAAGCCTGCAGTAAACTACATTTG NGG 10 8 17 8 24 0 100 5 10
    240605122940_rc9q ATAACATGGCAATAATGAAGTGCTGTTTCCTG(+CAGTGT)GTACCTTGGGAATACTAAAGAAAGAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605122938_gtiy GCAATGCTTTTTCTTTCTTTAGTATTCCCAAG(+CAGTGT)GTACCAGGAAACAGCACTTCATTATTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605122936_r2k1 TTAAAAGAGAAACCAGAAACATCTAATCTGAG(+CAGTGT)GATCCAGACAATAAATATTACATGGATCATT NGG 10 8 17 8 24 0 100 5 10
    240605122935_nyth CTCGGAATGATCCATGTAATATTTATTGTCTG(+CAGTGT)GATCCTCAGATTAGATGTTTCTGGTTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605122934_st0x TTAGATTATGTATGAAAATTCATACAATACTG(+CAGTGT)GATCCTATGACTAAAATGAAAATAGTAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605122933_9aaa TGCTGCTCCTACTATTTTCATTTTAGTCATAG(+CAGTGT)GATCCAGTATTGTATGAATTTTCATACATAA NGG 10 8 17 8 24 0 100 5 10
    240605122932_11yc GTCCCTGGTTCTGCTCAAACAGTTACTTCAAG(+CAGTGT)GTACCCTTAAAAGGGCTCCATTAAACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605122930_sbv5 GTACATTCTTGTTTAATGGAGCCCTTTTAAGG(+CAGTGT)GTACCTTGAAGTAACTGTTTGAGCAGAACCA NGG 10 8 17 8 24 0 100 5 10