Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123025_6mtr CACTGCCTCTCAGTAGTTTTAAAATATCCCAG(+CAGTGT)GTACCACGTGCTTACTTTTATTACTTGGGTA NGG 10 8 17 8 24 0 100 5 10
    240605123023_erpd TATATTACCCAAGTAATAAAAGTAAGCACGTG(+CAGTGT)GTACCTGGGATATTTTAAAACTACTGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605123022_xxj2 ACACGTAGTGATGATAGAAATTAGAATTTAAG(+CAGTGT)GATCCTATTGCTATGACTCTCATTTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605123021_0tt4 AGGGCAGAGAGAAATGAGAGTCATAGCAATAG(+CAGTGT)GATCCTTAAATTCTAATTTCTATCATCACTA NGG 10 8 17 8 24 0 100 5 10
    240605123019_huqf CATTGCTGTGTTCGAATAAAGCAAGCCACAGG(+CAGTGT)GTACCGTGAGGGAAAGCGTGAGGCACGCCGC NGG 10 8 17 8 24 0 100 5 10
    240605123018_66ob GTTCTGCGGCGTGCCTCACGCTTTCCCTCACG(+CAGTGT)GTACCCTGTGGCTTGCTTTATTCGAACACAG NGG 10 8 17 8 24 0 100 5 10
    240605123015_21t9 CACCAAGCCTGATAGCCTGCATTCGATCCCGG(+CAGTGT)GATCCATGTGGTACAAGAAGAGAGTTAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605123014_b7hk AGTCAATTCTAACTCTCTTCTTGTACCACATG(+CAGTGT)GATCCCGGGATCGAATGCAGGCTATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605123013_v7tz GTTGACACGTGCTGCTGTGTTTATTTAATATG(+CAGTGT)GATCCACATCAGGAAAGAGGGAATAATACAC NGG 10 8 17 8 24 0 100 5 10
    240605123012_sy46 AAAAAGTGTATTATTCCCTCTTTCCTGATGTG(+CAGTGT)GATCCATATTAAATAAACACAGCAGCACGTG NGG 10 8 17 8 24 0 100 5 10