Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123125_jgvr TATTGCTGTGTGTGCAGGACAGATGTTACCAG(+CAGTGT)GTACCCTCAAATCATTCTCTACCTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605123123_ck0t GCTTCAAAAATAAGGTAGAGAATGATTTGAGG(+CAGTGT)GTACCTGGTAACATCTGTCCTGCACACACAG NGG 10 8 17 8 24 0 100 5 10
    240605123121_a1za AGCACCTTCCTGGAGCCCCGTGCCCTTGCTGG(+CAGTGT)GATCCTGAGACAACTGTCAGCTGAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605123120_qh06 TTCCCACAGGGCTCAGCTGACAGTTGTCTCAG(+CAGTGT)GATCCCAGCAAGGGCACGGGGCTCCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605123118_19fj ACAGACCAGGCAGGGCTGGCTGGAGTTGAGGG(+CAGTGT)GATCCCGGTCCAAGAGAGAGAGAGCGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605123116_ixzq CAGCGCCCAGCGCTCTCTCTCTCTTGGACCGG(+CAGTGT)GATCCCCTCAACTCCAGCCAGCCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605123115_q6c6 CGAGAGGGCGTCAAAGAGGAAGCCTGGGCTGG(+CAGTGT)GATCCTTAGGCTCAGCTGTCTGTCTAATCCC NGG 10 8 17 8 24 0 100 5 10
    240605123113_9ptt GCTCTGGGATTAGACAGACAGCTGAGCCTAAG(+CAGTGT)GATCCCAGCCCAGGCTTCCTCTTTGACGCCC NGG 10 8 17 8 24 0 100 5 10
    240605123111_bapb GCTGTCTTTAGACACACCAGAAGAGGACATTG(+CAGTGT)GATCCATTACAGATGGTTGTGGTTGCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605123110_ua1q TCAAATCCCAGCAACCACAACCATCTGTAATG(+CAGTGT)GATCCAATGTCCTCTTCTGGTGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10