Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123054_ixcu GCCATTTCTGTACACACTGTTCTCTTGTTCAG(+CAGTGT)GTACCTGGAGAGGAAGGCTAGCCTTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605123052_cb0l GACTGGAGAAGAAGGCTAGCCTTCCTCTCCAG(+CAGTGT)GTACCTGAACAAGAGAACAGTGTGTACAGAA NGG 10 8 17 8 24 0 100 5 10
    240605123051_3t0t GTCTGCGAGTGTGGCCGCAAGGAAAATGGGAG(+CAGTGT)GATCCAACAGTCCTCTTTATTCTTTTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605123050_eyfx GCCAGGAAGCAAAAGAATAAAGAGGACTGTTG(+CAGTGT)GATCCTCCCATTTTCCTTGCGGCCACACTCG NGG 10 8 17 8 24 0 100 5 10
    240605123048_1cpi GGTTAGGAAATGATATGGTCAAAGCCAAAGTG(+CAGTGT)GATCCAACTTATTTTAAGTCCTTTTAAATGA NGG 10 8 17 8 24 0 100 5 10
    240605123047_lfj2 TACCTTCATTTAAAAGGACTTAAAATAAGTTG(+CAGTGT)GATCCACTTTGGCTTTGACCATATCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240605123046_3pqd ACTAGGCTGCCTGGAGTCCACAGGCAGTTCTG(+CAGTGT)GATCCACCATGTAAACGACAGAAGGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240605123044_kyan TTGTCAAGGTTCCTTCTGTCGTTTACATGGTG(+CAGTGT)GATCCAGAACTGCCTGTGGACTCCAGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605123043_5io2 TAAGGCACATGGAAGACTGTTTATTTGCACAG(+CAGTGT)GTACCGAAATTTTTTTAAGAACAGCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605123042_ut8w GAGATTTGACAGCTGTTCTTAAAAAAATTTCG(+CAGTGT)GTACCTGTGCAAATAAACAGTCTTCCATGTG NGG 10 8 17 8 24 0 100 5 10