Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122957_9kq2 ATGGGAAAGTCAGGGGAGAACTGGGGCTAGAG(+CAGTGT)GTACCCTGTAAACCAGTTTCTAGATAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605122955_vqn6 GAGAAGTTTTTATCTAGAAACTGGTTTACAGG(+CAGTGT)GTACCTCTAGCCCCAGTTCTCCCCTGACTTT NGG 10 8 17 8 24 0 100 5 10
    240605122954_z2cz AAAGTAAGCAAATTGTAGCATAAGATATATTG(+CAGTGT)GATCCACTCAGGAAGGTTGTATTACATTAGG NGG 10 8 17 8 24 0 100 5 10
    240605122953_lnda TATTTCCTAATGTAATACAACCTTCCTGAGTG(+CAGTGT)GATCCAATATATCTTATGCTACAATTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605122951_c4ip GGAACAAAGCACCATTGGCCTCTAGCTCTGGG(+CAGTGT)GATCCTACAGCGTCACCTTCCTCCTAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605122950_ll8f TCCTGCTGTCTAGGAGGAAGGTGACGCTGTAG(+CAGTGT)GATCCCCAGAGCTAGAGGCCAATGGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605122949_rfwe AAGGTTTGCCCTTTAGTTTAGAAACCAGATAG(+CAGTGT)GATCCCATCCTTGAATTTTAAATGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605122947_ynvo TGCAAATGACTTCATTTAAAATTCAAGGATGG(+CAGTGT)GATCCTATCTGGTTTCTAAACTAAAGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605122946_c8z5 GCAGTGCTGGTAGAATCCAGTAGAGGATATTG(+CAGTGT)GATCCTCTGTGACTAGAATTACAGATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605122945_w3gq GTTCATAACTATCTGTAATTCTAGTCACAGAG(+CAGTGT)GATCCAATATCCTCTACTGGATTCTACCAGC NGG 10 8 17 8 24 0 100 5 10