Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123213_720u GTCCCACTTTCAATGGGAGCTATGAGGTATGG(+CAGTGT)GTACCAGGCTTAGCAAGCAAGTTGTGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605123211_3u81 CTGTGGGGACCACAACTTGCTTGCTAAGCCTG(+CAGTGT)GTACCCATACCTCATAGCTCCCATTGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605123210_rxd8 TTGTTCAGGGACTACTCAAGAAAATATCTTTG(+CAGTGT)GATCCTTAGTTGGCCTTCAAGTCTATACATA NGG 10 8 17 8 24 0 100 5 10
    240605123208_x29z TCTTATATGTATAGACTTGAAGGCCAACTAAG(+CAGTGT)GATCCAAAGATATTTTCTTGAGTAGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605123206_mmf5 ACACAGTTTGAAGCACTATGTTTCTTCCCCTG(+CAGTGT)GTACCCTCTCTGTTCACAACTGCAGAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605123204_y5lm GAGAGAGGCTTCTGCAGTTGTGAACAGAGAGG(+CAGTGT)GTACCAGGGGAAGAAACATAGTGCTTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605123202_i7gr GTCATTGATGGGACAGGACAGGGAGATGGGAG(+CAGTGT)GATCCCCCAAACACTAGGAAGCAAGCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605123200_hrdp TGTTTCTGCTGCTTGCTTCCTAGTGTTTGGGG(+CAGTGT)GATCCTCCCATCTCCCTGTCCTGTCCCATCA NGG 10 8 17 8 24 0 100 5 10
    240605123158_hsll TCATCTGGTTGAAAACAGAAATCCAGAACCTG(+CAGTGT)GATCCCTAGGTCTTAGGGCTGACCCACACCA NGG 10 8 17 8 24 0 100 5 10
    240605123155_7jyt GAACATGGTGTGGGTCAGCCCTAAGACCTAGG(+CAGTGT)GATCCAGGTTCTGGATTTCTGTTTTCAACCA NGG 10 8 17 8 24 0 100 5 10