Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123315_ggd4 GAATCAGATAACAGAATCAGTGTTTCAGTCAG(+CAGTGT)GATCCACACTGCCTGCCAGGCTGTGCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605123314_mu4n CACATCCACAGCACAGCCTGGCAGGCAGTGTG(+CAGTGT)GATCCTGACTGAAACACTGATTCTGTTATCT NGG 10 8 17 8 24 0 100 5 10
    240605123312_xc65 CGGTGAAGCCTTACGACTCCACCACAGTTCGG(+CAGTGT)GATCCTAGATTCTGGCGTACATAGCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605123311_mx49 AGAGTTGCAGAGCTATGTACGCCAGAATCTAG(+CAGTGT)GATCCCGAACTGTGGTGGAGTCGTAAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605123309_v39r TGGTGCCATCCTAGGGACCAAGGTCTTTACAG(+CAGTGT)GTACCTGGGCCTGTGGGAGACATTGTTTACA NGG 10 8 17 8 24 0 100 5 10
    240605123307_21k7 TTGAGTGTAAACAATGTCTCCCACAGGCCCAG(+CAGTGT)GTACCTGTAAAGACCTTGGTCCCTAGGATGG NGG 10 8 17 8 24 0 100 5 10
    240605123306_iyxr CGGCGTCCTTCACTGATCGTTCTGTTTTTGAG(+CAGTGT)GATCCCTGTGGCCCCGGGATCAGCCCATCAC NGG 10 8 17 8 24 0 100 5 10
    240605123304_hco0 AAGGAGTGATGGGCTGATCCCGGGGCCACAGG(+CAGTGT)GATCCTCAAAAACAGAACGATCAGTGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605123303_6fr8 CTAAGTAGCAGCCCTGACTTCTGGTCAGGTAG(+CAGTGT)GATCCAACTGGCAGGAACCAGGCCAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605123301_kwrg ATGTGACTCCTTGGCCTGGTTCCTGCCAGTTG(+CAGTGT)GATCCTACCTGACCAGAAGTCAGGGCTGCTA NGG 10 8 17 8 24 0 100 5 10