Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123922_tdei TTTTGAAATCACAGCTCCAAGCTGCTGCCACG(+CAGTGT)GATCCACCAAACTGCTTTTGGCTTCCACGGG NGG 10 8 17 8 24 0 100 5 10
    240605123920_733o GAGGGACCATTTTCCTGACTGTACCTCTTTGG(+CAGTGT)GTACCCCATTCCTAAAGCAATAACTCAACGG NGG 10 8 17 8 24 0 100 5 10
    240605123918_758k CATTACCGTTGAGTTATTGCTTTAGGAATGGG(+CAGTGT)GTACCCAAAGAGGTACAGTCAGGAAAATGGT NGG 10 8 17 8 24 0 100 5 10
    240605123917_swlg CGTCCCTAGAAGCACAGGGCCCCTAGAAGCAG(+CAGTGT)GTACCAGCTCTGAGAACTTCCTGGCTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605123916_7l8c TGCACCAAGGAGCCAGGAAGTTCTCAGAGCTG(+CAGTGT)GTACCTGCTTCTAGGGGCCCTGTGCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605123915_11in TTCTCATTTGGGCTGGGGTGCCTATTTGTTTG(+CAGTGT)GTACCCCTCTGCTAAGTCACAAAGCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605123913_5y92 TACCCACAAAAGCTTTGTGACTTAGCAGAGGG(+CAGTGT)GTACCAAACAAATAGGCACCCCAGCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605123911_qvhd TTTGGTTTTTGCTGCTTGAGACCTTCTTTTGG(+CAGTGT)GTACCATTCACAGAGGACACTGAGGTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605123910_ylzh GAAGCCACCAACCTCAGTGTCCTCTGTGAATG(+CAGTGT)GTACCCAAAAGAAGGTCTCAAGCAGCAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605123908_f4gj TAAGTTCCCCTTAGCCTTGTGAGCCTTACTGG(+CAGTGT)GATCCAGTCCCCATATAGCATCCCTCCAACT NGG 10 8 17 8 24 0 100 5 10