Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123516_nx61 AGACCTGCATATGGGGAGGTAACATTGTTGAG(+CAGTGT)GATCCCACTCTGGCATTCATGTTGTCGTAAA NGG 10 8 17 8 24 0 100 5 10
    240605123514_hai3 CTTACTTTACGACAACATGAATGCCAGAGTGG(+CAGTGT)GATCCTCAACAATGTTACCTCCCCATATGCA NGG 10 8 17 8 24 0 100 5 10
    240605123511_klvs CACTTGAGTAGAGATGCCCTGGGAGGCCAGGG(+CAGTGT)GATCCCAAGGCCCCATCAGAATCTTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605123509_nzy8 AAACAAAAAACAAGATTCTGATGGGGCCTTGG(+CAGTGT)GATCCCCTGGCCTCCCAGGGCATCTCTACTC NGG 10 8 17 8 24 0 100 5 10
    240605123507_0kqt TCACCTTCCCTTCTGAATCCGTTGGGGCTGGG(+CAGTGT)GTACCACCTGGATTAAGACATTTAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240605123505_pzi8 ATTCCAAGATTTTAAATGTCTTAATCCAGGTG(+CAGTGT)GTACCCCAGCCCCAACGGATTCAGAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605123503_90ec TTAGGGGAAGAGACAAACTGTTCTGTACACTG(+CAGTGT)GTACCTTCTGAGTCCTGAACATTCTGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605123502_7e4x AAAATGTTTCCAGAATGTTCAGGACTCAGAAG(+CAGTGT)GTACCAGTGTACAGAACAGTTTGTCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605123501_8mbw TTCCTTGGTATGTTCTGTTCATCACATTCTAG(+CAGTGT)GATCCCACACCAAACATCCCACGTTCTCACT NGG 10 8 17 8 24 0 100 5 10
    240605123459_8w5t AGATTAGTGAGAACGTGGGATGTTTGGTGTGG(+CAGTGT)GATCCTAGAATGTGATGAACAGAACATACCA NGG 10 8 17 8 24 0 100 5 10