Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123559_8ahv AACTAAGAAACTTGCACTTACATGTCGAATGG(+CAGTGT)GTACCATCTAATAGCTAACACCCTGTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605123558_29ac TTGGCCTGCTACAGGGTGTTAGCTATTAGATG(+CAGTGT)GTACCCATTCGACATGTAAGTGCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605123556_fv08 GACAAGCAAAGGAGCTGGGGAGTGGAGTGTAG(+CAGTGT)GTACCATCACCTGAAAGCCACAGTCCTGACA NGG 10 8 17 8 24 0 100 5 10
    240605123555_ypch TTGTCTGTCAGGACTGTGGCTTTCAGGTGATG(+CAGTGT)GTACCTACACTCCACTCCCCAGCTCCTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605123554_2d3c TGTTCACAGCAGTAAAACCCTGACTGAGACAG(+CAGTGT)GTACCATCTAATAGCTAACACAATGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605123553_jez6 CTTGCCTGTGACATTGTGTTAGCTATTAGATG(+CAGTGT)GTACCTGTCTCAGTCAGGGTTTTACTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605123552_c4ib GGGACACAGAAACAGGGGAGCAGATGGGACAG(+CAGTGT)GATCCTTTCAGTCACCATCTACACCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605123550_q8q6 CACAACCTCCAGGTGTAGATGGTGACTGAAAG(+CAGTGT)GATCCTGTCCCATCTGCTCCCCTGTTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605123549_erth GTGCTCTCAGTCAACCACTGGACTAAGCACAG(+CAGTGT)GATCCCCAGTGGAGGAGTTAGAGAAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605123547_dsa1 CCTTCAGGCCTTTCTCTAACTCCTCCACTGGG(+CAGTGT)GATCCTGTGCTTAGTCCAGTGGTTGACTGAG NGG 10 8 17 8 24 0 100 5 10