Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123628_jxe1 CAGGAAGCTGGGAGCTTGGCATAGCTTATGGG(+CAGTGT)GTACCGAGTGTCCTGTGCTCTCCTCTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605123627_9jfs TCATGCTCAAAGAGGAGAGCACAGGACACTCG(+CAGTGT)GTACCCCATAAGCTATGCCAAGCTCCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605123625_yb9e TGTCACCAGCTGCCCTTCTCACCAGGAAACAG(+CAGTGT)GTACCTTTCCCTCTGCCTAGCGCCAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240605123624_gu9j CATTGTGGGGTTGGCGCTAGGCAGAGGGAAAG(+CAGTGT)GTACCTGTTTCCTGGTGAGAAGGGCAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605123623_o8m7 CAACCGGCCGGAGCAGCTGGGGCAGAGGATGG(+CAGTGT)GTACCACAGGTTGAGGGGATGAAGGTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605123621_iweg CCCTCATCAGACCTTCATCCCCTCAACCTGTG(+CAGTGT)GTACCCATCCTCTGCCCCAGCTGCTCCGGCC NGG 10 8 17 8 24 0 100 5 10
    240605123619_kadh AACCAGGCAGCGGCTCTCTCGATGACATTCAG(+CAGTGT)GATCCCTAAAGTCATATGCTTCCCACTATAG NGG 10 8 17 8 24 0 100 5 10
    240605123618_u0gz GGAAGCTATAGTGGGAAGCATATGACTTTAGG(+CAGTGT)GATCCTGAATGTCATCGAGAGAGCCGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605123617_p04j GGTAGATCTGGGAAAAGGTAGGGGAGGAATAG(+CAGTGT)GATCCAGATCAAAATACACTGTGAGTATGAA NGG 10 8 17 8 24 0 100 5 10
    240605123615_w4zx TATCATTCATACTCACAGTGTATTTTGATCTG(+CAGTGT)GATCCTATTCCTCCCCTACCTTTTCCCAGAT NGG 10 8 17 8 24 0 100 5 10