Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123716_xxwh GAAAACCACTAAGAATCTTCCAGCACTGTATG(+CAGTGT)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605123714_t5uh CATTTCCTTTACAATAAGATAGAAACATTGAG(+CAGTGT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605123713_ci4a AAAAACCACTAAGAATCTTCCAGCACTGTATG(+CAGTGT)GATCCTTAATGTCTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605123712_s3rz CATTTCCTTTACAATAAGATAGAGACATTAAG(+CAGTGT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605123710_jen3 CCAGGGTGACAACTTCATCTCCCATCCATGTG(+CAGTGT)GTACCAGTCTTACCATTCTCTTCATAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605123709_qzt5 ATCCAGAGCTTATGAAGAGAATGGTAAGACTG(+CAGTGT)GTACCACATGGATGGGAGATGAAGTTGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605123707_17co TTACATTAACAAGGCAAAATAAAGCCACAATG(+CAGTGT)GTACCATTAGGAAATTCCATCAAAACAACAG NGG 10 8 17 8 24 0 100 5 10
    240605123706_2kcb AAAGTCTGTTGTTTTGATGGAATTTCCTAATG(+CAGTGT)GTACCATTGTGGCTTTATTTTGCCTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605123705_3j8t TGGTGTGATAGTTTCTTAGGAGCAAACAACAG(+CAGTGT)GATCCTTTCTCCTAAACACAGGTTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240605123703_14r0 TGTTGAAAATTAAACCTGTGTTTAGGAGAAAG(+CAGTGT)GATCCTGTTGTTTGCTCCTAAGAAACTATCA NGG 10 8 17 8 24 0 100 5 10