Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123810_ufju ACTGAAAAGAAAAGGAATGAAGAAGACTTCTG(+CAGTGT)GATCCAAAGCCTTAAGAGAGTCTACGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605123809_xe8d TGTTAGCACACGTAGACTCTCTTAAGGCTTTG(+CAGTGT)GATCCAGAAGTCTTCTTCATTCCTTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605123808_1rau TGCGTTCCTGCAGTGTCCTCCTCGAGGAGCAG(+CAGTGT)GATCCACATTTTCAAGAATGTCCTGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240605123807_o6u3 TCACGAGGAGTCAGGACATTCTTGAAAATGTG(+CAGTGT)GATCCTGCTCCTCGAGGAGGACACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605123806_ayts AGCACATGTCAAGAAGGACTCAGGAAAGAAAG(+CAGTGT)GTACCTGTGCCTTGCCCCAGTGTCTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605123804_lyjx TGAACTCAGACAGACACTGGGGCAAGGCACAG(+CAGTGT)GTACCTTTCTTTCCTGAGTCCTTCTTGACAT NGG 10 8 17 8 24 0 100 5 10
    240605123803_us2d ATACCTTCCAAGGACCAGTAGACTTGAATACG(+CAGTGT)GATCCAATGGGAGTTGGTTGTCTGGTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605123801_3nl5 TCATCTTGACACCAGACAACCAACTCCCATTG(+CAGTGT)GATCCGTATTCAAGTCTACTGGTCCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605123759_dzbd GACTCCCTCCAGGGGTTCCCAGCTCCCACAGG(+CAGTGT)GTACCTGCCATCTCCATCCCTGGACTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605123758_h1c2 GTGGCTCTGCAGTCCAGGGATGGAGATGGCAG(+CAGTGT)GTACCCTGTGGGAGCTGGGAACCCCTGGAGG NGG 10 8 17 8 24 0 100 5 10