Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123859_qv6j GAATGTTAGATATTTTTTCCATGGGGTCAAAG(+CAGTGT)GTACCTAAGTATATGATTGCGAGTGGAGACA NGG 10 8 17 8 24 0 100 5 10
    240605123857_yljq CTGTGTATCTTTAAATACATCTGGATTGTGGG(+CAGTGT)GTACCTATGCGCAGTCTAGTTCAAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605123856_8l3i ATTTATTTATTTTTGAACTAGACTGCGCATAG(+CAGTGT)GTACCCCACAATCCAGATGTATTTAAAGATA NGG 10 8 17 8 24 0 100 5 10
    240605123855_m004 ACACTGTGCCCTAGTTCTGTGAGAATGAGCAG(+CAGTGT)GTACCTCTCTTCCCAGAGGCCATCACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605123854_bshg TCCCATCTAGGTGATGGCCTCTGGGAAGAGAG(+CAGTGT)GTACCTGCTCATTCTCACAGAACTAGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605123852_64y2 GGATTTCAAAGTGATGTCCGATGTAGCGCCAG(+CAGTGT)GTACCTTTGAATATCAATCAGAACCATTTAG NGG 10 8 17 8 24 0 100 5 10
    240605123851_fgqh CATATCTAAATGGTTCTGATTGATATTCAAAG(+CAGTGT)GTACCTGGCGCTACATCGGACATCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605123849_s1to TGTTTCGGGCAGTGCTCTCCCTAGTTTAAAAG(+CAGTGT)GTACCCAGGATCTTGTGTGGCTTGGATTTAA NGG 10 8 17 8 24 0 100 5 10
    240605123847_byls GGCATTTAAATCCAAGCCACACAAGATCCTGG(+CAGTGT)GTACCTTTTAAACTAGGGAGAGCACTGCCCG NGG 10 8 17 8 24 0 100 5 10
    240605123846_rcrh GAGTGAAAACAAGCTTTTCTTCAAATCTTCAG(+CAGTGT)GTACCTACCTCAGAATAGGTGTCATTAACGT NGG 10 8 17 8 24 0 100 5 10