Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123829_udlm CTGGTGCTTGGGGAGGCCAAAAGATGGCACTG(+CAGTGT)GATCCCCTGGGACTGGAGTTAAGAGATGGTA NGG 10 8 17 8 24 0 100 5 10
    240605123826_isv7 AGGAGGGTAGAATTTGAAAACCTCCTCCCGAG(+CAGTGT)GATCCAATGGTTCTTTCTGCAGAATGCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605123824_0slh GTAGGGCAGGCATTCTGCAGAAAGAACCATTG(+CAGTGT)GATCCTCGGGAGGAGGTTTTCAAATTCTACC NGG 10 8 17 8 24 0 100 5 10
    240605123821_gylf GGATGACAGAACCATTTCAGTGCAAACCCCAG(+CAGTGT)GATCCAAGGCTGCAAGCCTAAGTCACAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605123820_0l4y GGTCAGACTTGTGACTTAGGCTTGCAGCCTTG(+CAGTGT)GATCCTGGGGTTTGCACTGAAATGGTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605123817_6kut AGTCATGTTCTATTTCAGTTTTAAATGCCCAG(+CAGTGT)GTACCCAAGGAATGCACCCTTCTCCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605123815_7kaa GTGCGTGGCCAGGAGAAGGGTGCATTCCTTGG(+CAGTGT)GTACCTGGGCATTTAAAACTGAAATAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605123813_vwtf ACAACACAAGAAAACTTAAAAGAATGTGTATG(+CAGTGT)GTACCGTGGATCTTTCTGTGGTGGTTCATTC NGG 10 8 17 8 24 0 100 5 10
    240605123812_jkp1 CTGTGGAATGAACCACCACAGAAAGATCCACG(+CAGTGT)GTACCATACACATTCTTTTAAGTTTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605123810_ufju ACTGAAAAGAAAAGGAATGAAGAAGACTTCTG(+CAGTGT)GATCCAAAGCCTTAAGAGAGTCTACGTGTGC NGG 10 8 17 8 24 0 100 5 10