Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123759_dzbd GACTCCCTCCAGGGGTTCCCAGCTCCCACAGG(+CAGTGT)GTACCTGCCATCTCCATCCCTGGACTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605123758_h1c2 GTGGCTCTGCAGTCCAGGGATGGAGATGGCAG(+CAGTGT)GTACCCTGTGGGAGCTGGGAACCCCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605123757_4gyw ATTTTGGAAGAACACAGAAAGTTTTCAGACAG(+CAGTGT)GATCCATTTCCCCAGTACTCAACCAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240605123755_9j2y CTTTCCAATTTTGGTTGAGTACTGGGGAAATG(+CAGTGT)GATCCTGTCTGAAAACTTTCTGTGTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605123753_aiqd CCAAGAGGATGGGATTCATATTTAGTCCCTAG(+CAGTGT)GTACCGTTAAGAAGTGGCACACTATTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605123752_fg8e ATATACTTACAATAGTGTGCCACTTCTTAACG(+CAGTGT)GTACCTAGGGACTAAATATGAATCCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605123750_qaen TCATGCAGTGTCACCCGTGTCACGCAGCGCGG(+CAGTGT)GTACCCTGAGAGGACTCTGAATACCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605123749_inlh AGTTGGGGAAGGGTATTCAGAGTCCTCTCAGG(+CAGTGT)GTACCCGCGCTGCGTGACACGGGTGACACTG NGG 10 8 17 8 24 0 100 5 10
    240605123747_k0on TCATTTTGTTTCCTTTTAAGAAATTGCCACAG(+CAGTGT)GTACCCCTACGTTTAGTAACTACCATGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605123745_7bs3 ACTTATCAGCATGGTAGTTACTAAACGTAGGG(+CAGTGT)GTACCTGTGGCAATTTCTTAAAAGGAAACAA NGG 10 8 17 8 24 0 100 5 10