Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123743_q8mz TATATTTCCCTTTCCCATTCAATCAATAGGTG(+CAGTGT)GATCCAAGCTTGTTAGCTACTCAGGACTTGC NGG 10 8 17 8 24 0 100 5 10
    240605123742_5qg7 CTACTGCAAGTCCTGAGTAGCTAACAAGCTTG(+CAGTGT)GATCCACCTATTGATTGAATGGGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605123740_5znh CAAGTTCCAAGCCAACCAGGGGTACAAAATGG(+CAGTGT)GATCCTGACTTTAAAATTGAACAATTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605123739_gyt1 GTCATTTTTAAATTGTTCAATTTTAAAGTCAG(+CAGTGT)GATCCCATTTTGTACCCCTGGTTGGCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605123738_j27q TAAAATCTGAGGTAGGGAAAGGTAAAAAGCAG(+CAGTGT)GTACCCTCTCTTCAAATAGAAGCGCATGTTC NGG 10 8 17 8 24 0 100 5 10
    240605123736_d1gv ACAAGGAACATGCGCTTCTATTTGAAGAGAGG(+CAGTGT)GTACCTGCTTTTTACCTTTCCCTACCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605123735_ih4h AGTACTCACATTGCTACTGTCTTCTGTTGTAG(+CAGTGT)GTACCATGCTCGGAGCTTGCTTACAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605123734_mx40 AAATTTTTATTTGTAAGCAAGCTCCGAGCATG(+CAGTGT)GTACCTACAACAGAAGACAGTAGCAATGTGA NGG 10 8 17 8 24 0 100 5 10
    240605123732_eudb GACATTTTTACATTAGCAAAGTCACCTCATGG(+CAGTGT)GATCCTGCAGTTAAAGTGTTAAGACGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605123731_gybp TCTGCAGCCTCGTCTTAACACTTTAACTGCAG(+CAGTGT)GATCCCATGAGGTGACTTTGCTAATGTAAAA NGG 10 8 17 8 24 0 100 5 10