Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123844_lqiy TGAAAACGTTAATGACACCTATTCTGAGGTAG(+CAGTGT)GTACCTGAAGATTTGAAGAAAAGCTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605123843_srr3 CTCGAGGAATAGAAACACTGGATACTTAATGG(+CAGTGT)GTACCAGAAGAGTTTAGGAAACATGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605123841_5kj3 AGCCACTCCTCCATGTTTCCTAAACTCTTCTG(+CAGTGT)GTACCCATTAAGTATCCAGTGTTTCTATTCC NGG 10 8 17 8 24 0 100 5 10
    240605123840_omht TGGCAGTTTGATGGCAGTACTCTGTGTAGCAG(+CAGTGT)GATCCTGGATGAGACGAGGAGGAATCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605123838_ay4j CATGGCTGCAGATTCCTCCTCGTCTCATCCAG(+CAGTGT)GATCCTGCTACACAGAGTACTGCCATCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605123836_gipc GAGCTTAAAAGACACTCGTTAGAGTGCACCTG(+CAGTGT)GATCCTGCCATGACATCACAGGCGAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605123835_p6bz CGGCTGATGCTTCGCCTGTGATGTCATGGCAG(+CAGTGT)GATCCAGGTGCACTCTAACGAGTGTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605123834_zclh AAATGACCAGGCTCCTGGTAAATGGAAATCAG(+CAGTGT)GATCCAAGCAACTTTGAATTCTGGGAAATGA NGG 10 8 17 8 24 0 100 5 10
    240605123833_3m08 CAAAGTCATTTCCCAGAATTCAAAGTTGCTTG(+CAGTGT)GATCCTGATTTCCATTTACCAGGAGCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605123830_ekyz CTCACTACCATCTCTTAACTCCAGTCCCAGGG(+CAGTGT)GATCCAGTGCCATCTTTTGGCCTCCCCAAGC NGG 10 8 17 8 24 0 100 5 10