Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123913_5y92 TACCCACAAAAGCTTTGTGACTTAGCAGAGGG(+CAGTGT)GTACCAAACAAATAGGCACCCCAGCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605123911_qvhd TTTGGTTTTTGCTGCTTGAGACCTTCTTTTGG(+CAGTGT)GTACCATTCACAGAGGACACTGAGGTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605123910_ylzh GAAGCCACCAACCTCAGTGTCCTCTGTGAATG(+CAGTGT)GTACCCAAAAGAAGGTCTCAAGCAGCAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605123908_f4gj TAAGTTCCCCTTAGCCTTGTGAGCCTTACTGG(+CAGTGT)GATCCAGTCCCCATATAGCATCCCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240605123907_6m1v AAGGAAGTTGGAGGGATGCTATATGGGGACTG(+CAGTGT)GATCCCAGTAAGGCTCACAAGGCTAAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605123906_thnb ACTGAATACAGAACAGCGTGACTAGAAAGTTG(+CAGTGT)GATCCTGCGCTCTTGGACTGTTGGGAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605123905_2x7x TTTTTTCCTTTCCCAACAGTCCAAGAGCGCAG(+CAGTGT)GATCCAACTTTCTAGTCACGCTGTTCTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605123903_s2z9 GGAAATATGAGAGGACAGAAACTAAAAAATTG(+CAGTGT)GATCCAGTTCCAAGACAACTATTATCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605123902_csam ACAGCATCAAGATAATAGTTGTCTTGGAACTG(+CAGTGT)GATCCAATTTTTTAGTTTCTGTCCTCTCATA NGG 10 8 17 8 24 0 100 5 10
    240605123901_kh9t CCCTATGTCTCCACTCGCAATCATATACTTAG(+CAGTGT)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10