Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123943_xl3o AGGGGCCTCTCTCGTCCAGCATCCAGGGGTGG(+CAGTGT)GTACCTTGTTTCTTCTTTACTCATGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240605123941_ztnb TCCCATTAAGGAAAATGTGAAAATTGCATATG(+CAGTGT)GATCCACTCTCACCATAAAGGTGAGAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605123940_jcl6 CCTGCCTTCTTCTCACCTTTATGGTGAGAGTG(+CAGTGT)GATCCATATGCAATTTTCACATTTTCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605123939_ai21 AGTGTCTGGGTTTGCTGTCTGCAAGTGGAATG(+CAGTGT)GATCCCAAGGTGGGCCAGTGTCTTGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240605123937_w312 GGAAAGGCCATCAAGACACTGGCCCACCTTGG(+CAGTGT)GATCCATTCCACTTGCAGACAGCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605123935_5qye GAAAAACTGTTTATAAGAACCAAAATGATGTG(+CAGTGT)GATCCCTGTGTGTCCTGTGGAGGTGACATCA NGG 10 8 17 8 24 0 100 5 10
    240605123934_6t2m GAACTTGATGTCACCTCCACAGGACACACAGG(+CAGTGT)GATCCACATCATTTTGGTTCTTATAAACAGT NGG 10 8 17 8 24 0 100 5 10
    240605123933_tokv ATCAACAGCAAACTTAAAGGAAAAAGGAGCTG(+CAGTGT)GTACCCCAATGTCCTGACCTGGAGTCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605123931_p4d3 GCCAAATCTTGACTCCAGGTCAGGACATTGGG(+CAGTGT)GTACCAGCTCCTTTTTCCTTTAAGTTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605123929_h882 GCTACCTTTTAGGACTGAAGTTTTAAGCACTG(+CAGTGT)GTACCCACTGTGGGGCAAACCTGTGCTGCAG NGG 10 8 17 8 24 0 100 5 10