Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124030_bb3u TGTGACAGTTTCTACTACATGAGCACACAAAG(+CAGTGT)GTACCTGGTAGGGGTCTTTGTCTATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605124028_lm03 ACCCAGTAGCACCCGACTGTAGTGTGATTGGG(+CAGTGT)GTACCCTCTCCAGATTCTCTATCTTAACCAC NGG 10 8 17 8 24 0 100 5 10
    240605124027_nlts ACAAGGTGGTTAAGATAGAGAATCTGGAGAGG(+CAGTGT)GTACCCCAATCACACTACAGTCGGGTGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605124024_yh8v TTCTGCCCCCTTTGCCTGACCCCCAAGCCTGG(+CAGTGT)GTACCGCAAACACCAAGGCCTCCAGACAGTG NGG 10 8 17 8 24 0 100 5 10
    240605124023_oqim CCCTGCACTGTCTGGAGGCCTTGGTGTTTGCG(+CAGTGT)GTACCCAGGCTTGGGGGTCAGGCAAAGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605124021_hg8v AGACAATTCCTGTTCAGAGACAACAGGGGCCG(+CAGTGT)GTACCACTGGGAGATGGCAAAAGTTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605124019_re65 GGGTGGGAAGAAACTTTTGCCATCTCCCAGTG(+CAGTGT)GTACCGGCCCCTGTTGTCTCTGAACAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605124018_1jb3 GCAAGAGTCGGTTCCCTCCTTCTACTGTGTAG(+CAGTGT)GTACCAGTGATTAAACAGGCCGTTGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605124017_q5ye GCCTCCGAGCTCAACGGCCTGTTTAATCACTG(+CAGTGT)GTACCTACACAGTAGAAGGAGGGAACCGACT NGG 10 8 17 8 24 0 100 5 10
    240605124015_0g31 TATCACCACCCTGTGCAGTCAGAGAGGTCTGG(+CAGTGT)GATCCGGTCTCTACTCTGCATCTGGTGATCC NGG 10 8 17 8 24 0 100 5 10