Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124102_u0nr TTTATAGCTGAAATCTACTGTATTCTGGGGAG(+CAGTGT)GTACCCTGCCGACCCCTGGTCCCCCGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605124100_djk9 GCGAGGCCGCAGTCGCCCTCGGGGTTCTTAGG(+CAGTGT)GATCCCCAGCCTCTTGACAACAAGGCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605124058_u1kt GGGCCTGCCCGCCTTGTTGTCAAGAGGCTGGG(+CAGTGT)GATCCCTAAGAACCCCGAGGGCGACTGCGGC NGG 10 8 17 8 24 0 100 5 10
    240605124057_zktg GAAGAGCAGCTTGCCACAGCTGGCCTACAATG(+CAGTGT)GTACCAGCATTCTCTTCGTGATCTCAACACA NGG 10 8 17 8 24 0 100 5 10
    240605124055_3iqi CACAGTGTGTTGAGATCACGAAGAGAATGCTG(+CAGTGT)GTACCATTGTAGGCCAGCTGTGGCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605124053_rwzv GCACAGACCTAGGGATGCACTCCCTGCCTTTG(+CAGTGT)GATCCCCACTCTACCATTTGCTAATTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605124052_15co AAGGTCAACTAATTAGCAAATGGTAGAGTGGG(+CAGTGT)GATCCAAAGGCAGGGAGTGCATCCCTAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605124050_ejkt TCAAATCCAAAGCCGGCCTGTGTGATGTAGTG(+CAGTGT)GTACCTCTTTCCATCTCAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605124049_zbh8 GACTTTTTTTTTTTTTTTTGAGATGGAAAGAG(+CAGTGT)GTACCACTACATCACACAGGCCGGCTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605124048_n155 GAGGAGAGAAGACACTGGGGGCCTGGAGCTGG(+CAGTGT)GTACCAGGGCTACTACTGCCTTCCACTGTGA NGG 10 8 17 8 24 0 100 5 10