Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124151_iy8j TCCCGTGCTGGGCATCTCCTCATGGGTGGCAG(+CAGTGT)GATCCTGAATAGATGGGGCTGCAGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605124150_lsxy GCTGCAGCCCCTGCAGCCCCAACCGCAAGCTG(+CAGTGT)GATCCGGGATCTGCACATCAGCAGCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605124147_8hf2 GGGCTCCAAAGGCTGCTGATGTGCAGATCCCG(+CAGTGT)GATCCAGCTTGCGGTTGGGGCTGCAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605124146_c365 ACACTAAATAAAACCAAGCCAATAGTCTTGTG(+CAGTGT)GATCCCTGAATATTTCTCATTCCACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240605124144_ash1 TAAGATGAAGTGTGGAATGAGAAATATTCAGG(+CAGTGT)GATCCACAAGACTATTGGCTTGGTTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605124143_k1zx CTTGCCTGTCTGGTCAGCAGTATTGCACACAG(+CAGTGT)GATCCACAGATGACAGTTGATGGCTCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605124142_7l0i GGTGGGGGGAGAGCCATCAACTGTCATCTGTG(+CAGTGT)GATCCTGTGTGCAATACTGCTGACCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605124140_l2qv TCTTAGGAGAGCAAGGATATGGAGTCATTGTG(+CAGTGT)GATCCCTGCCTGTGGGGGGTTTGTGAGATAA NGG 10 8 17 8 24 0 100 5 10
    240605124139_wqe8 TGCTTTTATCTCACAAACCCCCCACAGGCAGG(+CAGTGT)GATCCACAATGACTCCATATCCTTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605124137_nixm ACATGCTGGGAGAAAATTAACACTAAAATCTG(+CAGTGT)GTACCAGCCAGAGGAAGGAACTTGACTCAAA NGG 10 8 17 8 24 0 100 5 10