Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124238_3u6b CTCCCCCTGAGAAAGGCTCAATGCTACACTGG(+CAGTGT)GATCCTGAAGACCCAGTGGAGTCATCAACCA NGG 10 8 17 8 24 0 100 5 10
    240605124236_hctg CGGCCTCTTCAGTCTGAGAAGGAAGCCTCAGG(+CAGTGT)GATCCACAATCCAGGGACCCTCACCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605124235_w9s6 AAGCATGGGAGGGTGAGGGTCCCTGGATTGTG(+CAGTGT)GATCCCTGAGGCTTCCTTCTCAGACTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605124234_jrw0 ACTTGTTTGTTTTCCTTTTTGAGTTCACGTTG(+CAGTGT)GATCCTTTCTTGTACAACTTCTGCGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605124232_2bkk CTGTGTCCTTCCGCAGAAGTTGTACAAGAAAG(+CAGTGT)GATCCAACGTGAACTCAAAAAGGAAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240605124231_eu1o AAGAGGCCCACTAGAGGGCCCATCAGACAGTG(+CAGTGT)GTACCAGTGAACTCCCTGAAGGATGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605124230_uui1 CACCACACCTCCATCCTTCAGGGAGTTCACTG(+CAGTGT)GTACCACTGTCTGATGGGCCCTCTAGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605124227_rbrq AGATGCACAATCCACAGTACTTGGGGCCTGGG(+CAGTGT)GTACCTATGGGAAATAAACAATATAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605124226_0kp2 CCATAGAAAACTATATTGTTTATTTCCCATAG(+CAGTGT)GTACCCCAGGCCCCAAGTACTGTGGATTGTG NGG 10 8 17 8 24 0 100 5 10
    240605124225_8tm7 TCTGGATGTCACCATGTGTTTCTCAAAATCTG(+CAGTGT)GTACCCTCCCCTCTCAGAGATTACTGACTTC NGG 10 8 17 8 24 0 100 5 10