Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124324_syx9 TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+CAGTGT)GTACCCAGATAGGCAAGAAGGCTTCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240605124323_9gm2 GGTGACCTCAAAGGTATCCCATGATCAGCATG(+CAGTGT)GTACCAGCAGTAGTGAGTAACCAGTTCTAGT NGG 10 8 17 8 24 0 100 5 10
    240605124322_vc9j GGGAAACTAGAACTGGTTACTCACTACTGCTG(+CAGTGT)GTACCATGCTGATCATGGGATACCTTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605124321_vivo ACGTGATGGTAGGCTTGTGGGGTGACTGCTAG(+CAGTGT)GTACCTCTAGAGGGCAACACTTACACAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605124319_o7tz CTGTTTTCCTGTGTAAGTGTTGCCCTCTAGAG(+CAGTGT)GTACCTAGCAGTCACCCCACAAGCCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240605124317_nqyb CAGGTGGTCTGACATCCTCTTGTGCCTCCCTG(+CAGTGT)GTACCTGCAGACACATTCTCTCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605124316_ajlb GAGAGAGACAGAGAGAGAGAATGTGTCTGCAG(+CAGTGT)GTACCAGGGAGGCACAAGAGGATGTCAGACC NGG 10 8 17 8 24 0 100 5 10
    240605124315_tgan GTGTGCACTGCTTGTATGCGTGTGGATGTGTG(+CAGTGT)GATCCCACCTGCCGCCTCCCCATCTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605124313_auq2 ACACCCAGAGCAGATGGGGAGGCGGCAGGTGG(+CAGTGT)GATCCACACATCCACACGCATACAAGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605124311_p3mj TCTGGGTGGGCGAGATACCCAATGCCAGCCAG(+CAGTGT)GTACCAGCGCCCCAGGAGCATGAAATGCCAG NGG 10 8 17 8 24 0 100 5 10