Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124854_bjm1 CTGCAGGGGCATTTCTCAAAGGAGAAGAGATG(+CAGTGT)GTACCTCCCACTGGAACCAGACAGACCACCA NGG 10 8 17 8 24 0 100 5 10
    240605124852_s8p9 TCTGGGGTAGCTTACCCAGTGCTAGGCACTGG(+CAGTGT)GATCCTGAAAGTACCTTTCTTGGTGATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605124851_mvoo CTGTTCCAGATCACCAAGAAAGGTACTTTCAG(+CAGTGT)GATCCCAGTGCCTAGCACTGGGTAAGCTACC NGG 10 8 17 8 24 0 100 5 10
    240605124849_99vt GAAGGCAGGTCCCCAGTTGTTGGGCCAGCAGG(+CAGTGT)GTACCTTCCTTCTTCTGCAGACTGCGATAGT NGG 10 8 17 8 24 0 100 5 10
    240605124848_9ra3 GTCACACTATCGCAGTCTGCAGAAGAAGGAAG(+CAGTGT)GTACCCTGCTGGCCCAACAACTGGGGACCTG NGG 10 8 17 8 24 0 100 5 10
    240605124846_zcfl ACGCCTTTAGTCTCCGGATGTCTCAAATTATG(+CAGTGT)GATCCTGAGCGCAGCCGTCTGCTCTTTCATG NGG 10 8 17 8 24 0 100 5 10
    240605124845_ph4z TTATGCATGAAAGAGCAGACGGCTGCGCTCAG(+CAGTGT)GATCCATAATTTGAGACATCCGGAGACTAAA NGG 10 8 17 8 24 0 100 5 10
    240605124844_q42q CCTGTGTCTCAGGGACGCCTCTACTTTCTTGG(+CAGTGT)GATCCGGGACCGTTCTCAGAGGCACTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605124841_ej4v TTCCGTCCCCAGTGCCTCTGAGAACGGTCCCG(+CAGTGT)GATCCCAAGAAAGTAGAGGCGTCCCTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605124840_ebw7 CAGCAAGATGCTCCTGATGCCCTTTGGTCCAG(+CAGTGT)GATCCACTGCCTCCTTGTTCTAGAGGCTCAG NGG 10 8 17 8 24 0 100 5 10