Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124223_tq7r TGGCAGAAGTCAGTAATCTCTGAGAGGGGAGG(+CAGTGT)GTACCAGATTTTGAGAAACACATGGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240605124220_0c52 TTCCTCTTCTCCTCCCCATGGAAGGGGGGGGG(+CAGTGT)GATCCCTGGCTCAGCATCCACTGCTGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605124218_spx5 AGGTTTCCACCAGCAGTGGATGCTGAGCCAGG(+CAGTGT)GATCCCCCCCCCCTTCCATGGGGAGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605124216_10wv TACCTGCCTCTCAAGTGCTGGAATTAAAGGCG(+CAGTGT)GTACCAGCAGCCCGGCTCCTTTGTTCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605124215_e6zy ATAACAAAAGGAACAAAGGAGCCGGGCTGCTG(+CAGTGT)GTACCGCCTTTAATTCCAGCACTTGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605124213_4xcp ATACCTTCAGAGACAAACATGTTTACACATAG(+CAGTGT)GTACCCGTGTGAGCCTAGATACACACATACA NGG 10 8 17 8 24 0 100 5 10
    240605124212_ewf7 TTTAGTGTATGTGTGTATCTAGGCTCACACGG(+CAGTGT)GTACCTATGTGTAAACATGTTTGTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605124211_3fch AGACTTAAGAGTAGGAAAGGGACTGGTACATG(+CAGTGT)GTACCTGCCAGCCCAGCCTAGCACGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605124209_ogdy AAAAAACTCTTCGTGCTAGGCTGGGCTGGCAG(+CAGTGT)GTACCATGTACCAGTCCCTTTCCTACTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605124207_gupd GCTGAGAATCACAGATTTAGCAGCTCCTAGGG(+CAGTGT)GTACCCACCACATTGAGCTATGCTGAATTAA NGG 10 8 17 8 24 0 100 5 10