Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124735_mkd5 ACCACTGGATTCAGCAACAAGATCTAGGCTGG(+CAGTGT)GTACCCCTGTTGTCCCTGCTTTACTCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605124733_fw67 TAGCAAACTCCCCAGTCCCCACAGCCACTCGG(+CAGTGT)GTACCTCTTTTCCATAGACAGTTCTGATCAC NGG 10 8 17 8 24 0 100 5 10
    240605124732_9roq CACACGTGATCAGAACTGTCTATGGAAAAGAG(+CAGTGT)GTACCCGAGTGGCTGTGGGGACTGGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605124730_ta4d TCTGGCATTTCATGTTACTGCAGGCCTTTGAG(+CAGTGT)GTACCTCAATGTGTAAGATATTTCTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605124729_tbmw TTAAGCCCCACAGAAATATCTTACACATTGAG(+CAGTGT)GTACCTCAAAGGCCTGCAGTAACATGAAATG NGG 10 8 17 8 24 0 100 5 10
    240605124728_tujz ATACTGAAACAGACTTCCAACAGGAAGGCACG(+CAGTGT)GTACCTGAGAGCAAGTGTTTCTGAATAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605124727_6yp7 GTCCAGTTCTATTCAGAAACACTTGCTCTCAG(+CAGTGT)GTACCGTGCCTTCCTGTTGGAAGTCTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605124725_j5tv TAAATGACAGTGTGTAACTATCTGTAACTGGG(+CAGTGT)GATCCCATGCCCTTTTCTGACCTTCAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605124723_j50s TTGGTGACCTTGAAGGTCAGAAAAGGGCATGG(+CAGTGT)GATCCCCAGTTACAGATAGTTACACACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605124722_lwh2 GCCGTTAGACTGCCTGCCTTTCAGAGGTCTGG(+CAGTGT)GATCCTGGCTCCCCACCCCCAAGTGCGAGAC NGG 10 8 17 8 24 0 100 5 10