Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124254_qmca GCTGTGGTATGGAAATCCCAGAGTGGGGCTAG(+CAGTGT)GATCCAGATGAGAGTCAGCTTTCTCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605124253_l1ln TAACTTGGTGATAGAGTGCTTGTCTGCCACAG(+CAGTGT)GATCCTAGATTCAATGCCCAGCACCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240605124251_murl TTTTGGCTTGTGGTGCTGGGCATTGAATCTAG(+CAGTGT)GATCCTGTGGCAGACAAGCACTCTATCACCA NGG 10 8 17 8 24 0 100 5 10
    240605124249_y9iu GAATATTATATTCATCTTCACATGGTCCTGGG(+CAGTGT)GATCCAAATGTCTAGTCTTTCTTTTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605124248_6weu TTCTTTAAAAAAAAAGAAAGACTAGACATTTG(+CAGTGT)GATCCCCAGGACCATGTGAAGATGAATATAA NGG 10 8 17 8 24 0 100 5 10
    240605124246_k6sq GGTAGCTGTGATCTTCCTTCATGGAAGTTCTG(+CAGTGT)GTACCAGACTCCAAGGGGGGGGGGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605124245_irof ATCTCTGCCTGCCCCCCCCCCCTTGGAGTCTG(+CAGTGT)GTACCAGAACTTCCATGAAGGAAGATCACAG NGG 10 8 17 8 24 0 100 5 10
    240605124243_rh8h CCAAGATGCCCCTTCTATGGTAACAGCCCAAG(+CAGTGT)GTACCTGGGTTATCTGGCCTGAGGCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240605124240_5qtw ATTCCTCTGTAGCCTCAGGCCAGATAACCCAG(+CAGTGT)GTACCTTGGGCTGTTACCATAGAAGGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605124239_5rhc TTAGCTGGTTGATGACTCCACTGGGTCTTCAG(+CAGTGT)GATCCCAGTGTAGCATTGAGCCTTTCTCAGG NGG 10 8 17 8 24 0 100 5 10