Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124309_h88e GTGGCCTGGCATTTCATGCTCCTGGGGCGCTG(+CAGTGT)GTACCTGGCTGGCATTGGGTATCTCGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605124308_dc7l GCTGTCTTCAGACACACCAGAACGGGGCATTG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605124306_swrd ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCAATGCCCCGTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605124305_odcb GTCCCCACTCCCCCGACCCAGCTCCACGGGAG(+CAGTGT)GATCCAGGAGGCAACCCCAGCCCAGCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605124303_l5ak GATGTCTCCAGCTGGGCTGGGGTTGCCTCCTG(+CAGTGT)GATCCTCCCGTGGAGCTGGGTCGGGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605124301_wu9w GCTGAACCCATCAGACGGGTCCGACTAGCCGG(+CAGTGT)GATCCGAGGCCTTGGCCCAGGAGATAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605124259_rhhn CCCCTATGCCTATCTCCTGGGCCAAGGCCTCG(+CAGTGT)GATCCCGGCTAGTCGGACCCGTCTGATGGGT NGG 10 8 17 8 24 0 100 5 10
    240605124258_y2ls TTGAAGTAATCGCCATCCTCCCAGCATAAATG(+CAGTGT)GATCCTTAGCAAGGCAATGTGGATGCAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605124257_ih99 TGGCAACTCTGCATCCACATTGCCTTGCTAAG(+CAGTGT)GATCCATTTATGCTGGGAGGATGGCGATTAC NGG 10 8 17 8 24 0 100 5 10
    240605124256_xzol GCCTAGAGGCAGAGAAAGCTGACTCTCATCTG(+CAGTGT)GATCCTAGCCCCACTCTGGGATTTCCATACC NGG 10 8 17 8 24 0 100 5 10