Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124910_ygc9 CTCCCTGGCATCCCAGGCCCATAGCTGGGCTG(+CAGTGT)GATCCTCCCTGCTACAAGACAATCTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605124908_th2t GAGACCCACCTGTCACCGTGTGTCATCAAGGG(+CAGTGT)GATCCGGTGGGGAAAAGAAGGGGAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240605124906_8tjv CTCACCTGTGTTTCCCCTTCTTTTCCCCACCG(+CAGTGT)GATCCCCTTGATGACACACGGTGACAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605124904_2zs7 GCTGAGTGAGGCCACTCCCATTGAAGAGTGAG(+CAGTGT)GATCCTGGAGCCCCAAAGGCCTTTGGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605124903_62mi TAGCTCATTCCCAAAGGCCTTTGGGGCTCCAG(+CAGTGT)GATCCTCACTCTTCAATGGGAGTGGCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605124901_14kg TCCCGTCATGTTTCTCCTTACTCATCCAATTG(+CAGTGT)GTACCCACAACTGAAGAGCTCGTGTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240605124900_zdtx GCCCGCCAAATACACGAGCTCTTCAGTTGTGG(+CAGTGT)GTACCAATTGGATGAGTAAGGAGAAACATGA NGG 10 8 17 8 24 0 100 5 10
    240605124858_3zp6 CTCTTTTGTCTGTGGAGTCTGCTGTGTGGCAG(+CAGTGT)GATCCCAGCCCTAGAGCTGCAGGGCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605124856_h0u1 CCCACAGGGCAGCCCTGCAGCTCTAGGGCTGG(+CAGTGT)GATCCTGCCACACAGCAGACTCCACAGACAA NGG 10 8 17 8 24 0 100 5 10
    240605124855_nsjj CTGGGTGGTGGTCTGTCTGGTTCCAGTGGGAG(+CAGTGT)GTACCATCTCTTCTCCTTTGAGAAATGCCCC NGG 10 8 17 8 24 0 100 5 10