Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124136_az3p CTTATTTTGAGTCAAGTTCCTTCCTCTGGCTG(+CAGTGT)GTACCAGATTTTAGTGTTAATTTTCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605124135_3j4p GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCCAGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605124133_t57o GGATGGACCATCTAGAGACTGCCATATCTGGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605124132_wmwm AGTGTTGGCCTATAGGCTGTACAAATATCAAG(+CAGTGT)GTACCCGGAGTAGTTACTCCTCTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605124129_sg61 TTTTTTCTGAAGAAGAGGAGTAACTACTCCGG(+CAGTGT)GTACCTTGATATTTGTACAGCCTATAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605124128_mkh4 AAGATGGGGCTCAAGAAATGGTCAGCTCTTGG(+CAGTGT)GTACCTAAGTATTTGAACAGAAGCTGCCACA NGG 10 8 17 8 24 0 100 5 10
    240605124126_mp1l GCTTTTGTGGCAGCTTCTGTTCAAATACTTAG(+CAGTGT)GTACCCAAGAGCTGACCATTTCTTGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605124124_fm4w CAAGCCTTCGGCAGAGGGTCATGTACCACTGG(+CAGTGT)GATCCCAAGTACGGCTAGACCCACCAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605124123_s44u TTGGCCTTTTTGGTGGGTCTAGCCGTACTTGG(+CAGTGT)GATCCCAGTGGTACATGACCCTCTGCCGAAG NGG 10 8 17 8 24 0 100 5 10
    240605124122_2qnz ATAAAGTTCAGTGCTATAGCACTTTGTGTGAG(+CAGTGT)GTACCTGGGTTCAACCCCAGCACTGCCTCAT NGG 10 8 17 8 24 0 100 5 10