Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124045_jghk TGGTCTCACAGTGGAAGGCAGTAGTAGCCCTG(+CAGTGT)GTACCCAGCTCCAGGCCCCCAGTGTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605124044_shlm GAGGCCAGCCTGGGTTAGAGTGACATGTAGTG(+CAGTGT)GATCCTCATTATGTGTTCATGTTAGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605124043_67uo TTGAGCTTTTTCTAACATGAACACATAATGAG(+CAGTGT)GATCCACTACATGTCACTCTAACCCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605124041_p59s CCATACCCTAAATGTATTCTATACTATACCTG(+CAGTGT)GTACCTCAGGGGGAAGGGATGCCAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605124039_8qqu GTGGACCCATGCTGGCATCCCTTCCCCCTGAG(+CAGTGT)GTACCAGGTATAGTATAGAATACATTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605124038_cr13 GCCTATGGATATATGCATGGAGGTGTGTCTGG(+CAGTGT)GTACCTATTCACACAGGCTTACATATGACCA NGG 10 8 17 8 24 0 100 5 10
    240605124036_9p8i GAGTGTGGTCATATGTAAGCCTGTGTGAATAG(+CAGTGT)GTACCCAGACACACCTCCATGCATATATCCA NGG 10 8 17 8 24 0 100 5 10
    240605124035_4o1e CGTTGGTCTTCAGTTCTGCAGGGAAGTTCTGG(+CAGTGT)GTACCTTTGGTTTTGTTTAAGGAGAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605124033_avxl ATATTTTTTTTTCTCCTTAAACAAAACCAAAG(+CAGTGT)GTACCCAGAACTTCCCTGCAGAACTGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605124031_c7pg TTCCACAAAGTATAGACAAAGACCCCTACCAG(+CAGTGT)GTACCTTTGTGTGCTCATGTAGTAGAAACTG NGG 10 8 17 8 24 0 100 5 10