Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605124013_yof6 CAGATGGATCACCAGATGCAGAGTAGAGACCG(+CAGTGT)GATCCCAGACCTCTCTGACTGCACAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605124012_z2le GGAACCCCTTGCACTGTGAAGCTAGAGCGTGG(+CAGTGT)GTACCTAACTCACCGTTGTCCCAGAATGAGG NGG 10 8 17 8 24 0 100 5 10
    240605124010_7jrg CCCCGCCTCATTCTGGGACAACGGTGAGTTAG(+CAGTGT)GTACCCACGCTCTAGCTTCACAGTGCAAGGG NGG 10 8 17 8 24 0 100 5 10
    240605124007_8bu4 TCTGCGTGGGAGAACTTGGTGTCTCACCCTGG(+CAGTGT)GTACCCATTCCTGGTGTATGGAAGCACCTCC NGG 10 8 17 8 24 0 100 5 10
    240605124006_mt5q GTGAAGGAGGTGCTTCCATACACCAGGAATGG(+CAGTGT)GTACCCAGGGTGAGACACCAAGTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605124004_2qxl ATGCAGATCAGTTCTGCACACCTCCAGGGGTG(+CAGTGT)GATCCAGCTGTAAGGCCATACCTATATCTTC NGG 10 8 17 8 24 0 100 5 10
    240605124003_plyi ATCTGGAAGATATAGGTATGGCCTTACAGCTG(+CAGTGT)GATCCACCCCTGGAGGTGTGCAGAACTGATC NGG 10 8 17 8 24 0 100 5 10
    240605124001_dwtz CACTGATTGAGAAGGAGAGTGTATAATTTAAG(+CAGTGT)GTACCTTAAGTTTAATCTTTGAAAGATTGCC NGG 10 8 17 8 24 0 100 5 10
    240605124000_iva5 AAGATGGCAATCTTTCAAAGATTAAACTTAAG(+CAGTGT)GTACCTTAAATTATACACTCTCCTTCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240605123959_rbcv GAAAACAGCATCAGAGTTCACTTCGCATCATG(+CAGTGT)GTACCCCAAGGGATGGGGATACGGGTATGAC NGG 10 8 17 8 24 0 100 5 10