Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123957_mznj TCAGTGTCATACCCGTATCCCCATCCCTTGGG(+CAGTGT)GTACCATGATGCGAAGTGAACTCTGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605123955_gn4z GAAGGCATACGGATGACACTGTGGTCACAAAG(+CAGTGT)GATCCCTTGCTTTGGCCTCAAACGAGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605123954_pfsw TTGGCACTGACTCGTTTGAGGCCAAAGCAAGG(+CAGTGT)GATCCTTTGTGACCACAGTGTCATCCGTATG NGG 10 8 17 8 24 0 100 5 10
    240605123952_kh1c GCGCAGCAATATTTAAAAACGAGAAGAACCAG(+CAGTGT)GTACCAAGATCATAGAAGAAATTATTATCTG NGG 10 8 17 8 24 0 100 5 10
    240605123951_hp26 GGAATCAGATAATAATTTCTTCTATGATCTTG(+CAGTGT)GTACCTGGTTCTTCTCGTTTTTAAATATTGC NGG 10 8 17 8 24 0 100 5 10
    240605123950_rcas GCCTTCATCCCTCACCTCAGAGCGAAACACTG(+CAGTGT)GATCCAGCCCCTTTGTCACCATCACTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605123948_jz5s GGCCTAGAAAAGTGATGGTGACAAAGGGGCTG(+CAGTGT)GATCCAGTGTTTCGCTCTGAGGTGAGGGATG NGG 10 8 17 8 24 0 100 5 10
    240605123947_uvy6 CTTCATATAGCCCAGGCTAGACCCAAACTCAG(+CAGTGT)GATCCTCCTGCCTCCACCTCCAGAGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605123946_6gf8 TAATCTCAACACTCTGGAGGTGGAGGCAGGAG(+CAGTGT)GATCCTGAGTTTGGGTCTAGCCTGGGCTATA NGG 10 8 17 8 24 0 100 5 10
    240605123944_n60v GTTTTGATTCTCATGAGTAAAGAAGAAACAAG(+CAGTGT)GTACCCACCCCTGGATGCTGGACGAGAGAGG NGG 10 8 17 8 24 0 100 5 10