Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123702_2f6c GCATGGGATGTCCTATTTTAGGAGGTTTCCAG(+CAGTGT)GATCCTAGCCCACTAAATTAACATCATGCCA NGG 10 8 17 8 24 0 100 5 10
    240605123700_9ias AAGCCTGGCATGATGTTAATTTAGTGGGCTAG(+CAGTGT)GATCCTGGAAACCTCCTAAAATAGGACATCC NGG 10 8 17 8 24 0 100 5 10
    240605123659_m21a CTAAGAGCATCATCTATTCTACTTGGGATAAG(+CAGTGT)GTACCTGGTAAAGCTCTAAGCTCTAAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605123657_8kw0 CCTTCTAACTTTAGAGCTTAGAGCTTTACCAG(+CAGTGT)GTACCTTATCCCAAGTAGAATAGATGATGCT NGG 10 8 17 8 24 0 100 5 10
    240605123656_uaj0 GAGACGCTCTTAGGGGGAGCACGACTTTCATG(+CAGTGT)GATCCTGGGGCCTGGGTGGGCTGGGGGACAG NGG 10 8 17 8 24 0 100 5 10
    240605123653_09ub CTCAGCTGTCCCCCAGCCCACCCAGGCCCCAG(+CAGTGT)GATCCATGAAAGTCGTGCTCCCCCTAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605123651_7i1n CATAATATAAGCACTTATATCCATAATTGTGG(+CAGTGT)GTACCAGTGCAAAACCATCTTGGTACATTTC NGG 10 8 17 8 24 0 100 5 10
    240605123650_ht2t ATCAGGAAATGTACCAAGATGGTTTTGCACTG(+CAGTGT)GTACCCACAATTATGGATATAAGTGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240605123648_ltub TGAAATTAAAGGCAGACCGGTTGGGTGCTAAG(+CAGTGT)GATCCCTGGTGTGGAGAGGCCATTTCATTGA NGG 10 8 17 8 24 0 100 5 10
    240605123646_n557 GCTGCTCAATGAAATGGCCTCTCCACACCAGG(+CAGTGT)GATCCTTAGCACCCAACCGGTCTGCCTTTAA NGG 10 8 17 8 24 0 100 5 10