Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123359_k9e0 GGATGGACCATCTAGTGACTGCCATATGCAGG(+CAGTGT)GATCCATCCCATAATTAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605123358_gzl3 GGTGTCAGCATTTGGAAGCTAATTATGGGATG(+CAGTGT)GATCCCTGCATATGGCAGTCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605123356_60cs AGACCAGTGTGGAAAGGACCGTGGGGTCCTTG(+CAGTGT)GATCCTCACTTCCTGATATCAAGCTACCACT NGG 10 8 17 8 24 0 100 5 10
    240605123355_7ig8 AGAACAGTGGTAGCTTGATATCAGGAAGTGAG(+CAGTGT)GATCCAAGGACCCCACGGTCCTTTCCACACT NGG 10 8 17 8 24 0 100 5 10
    240605123353_hpab CCACTTTATCATAGAAATACCCCACTCAGCTG(+CAGTGT)GTACCAATTTGCCTTAGTCATGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240605123352_szhu CCAGGAATAGAAACCATGACTAAGGCAAATTG(+CAGTGT)GTACCAGCTGAGTGGGGTATTTCTATGATAA NGG 10 8 17 8 24 0 100 5 10
    240605123351_f14c CTCACCTCCCAGGTCTCTGGTACATTCTGGAG(+CAGTGT)GATCCCTGCACCTCTTTCTTCCCAAGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605123349_al64 AACAAACAACCTTGGGAAGAAAGAGGTGCAGG(+CAGTGT)GATCCTCCAGAATGTACCAGAGACCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605123348_6ent CCACATTGTCACAGGAATGCCCTACTATGCTG(+CAGTGT)GTACCAATTTGTCTTAGTCAGGGTATCTATT NGG 10 8 17 8 24 0 100 5 10
    240605123346_ou76 CCAGGAATAGATACCCTGACTAAGACAAATTG(+CAGTGT)GTACCAGCATAGTAGGGCATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10