Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123300_yfcl GCTGTCTTCAGACACACCAGAAGAGGGCATTG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605123258_491j ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605123257_hl2o TTGATCCTGACTGTTCCTCAGAGAGGGCAACG(+CAGTGT)GATCCCTGCTCAGTTTGCTGGCTCTTGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605123255_nbri GAGAAGTGACAAGAGCCAGCAAACTGAGCAGG(+CAGTGT)GATCCGTTGCCCTCTCTGAGGAACAGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605123254_3v0n GGAGGTTCAGTGACCAGTAGGCCTCCCCACTG(+CAGTGT)GTACCACCACTTCCACTGTGAGACTGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605123252_5xib TTGAGTTCTGCAGTCTCACAGTGGAAGTGGTG(+CAGTGT)GTACCAGTGGGGAGGCCTACTGGTCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240605123251_liww AAGATTCTAAAGAGCGTTGAAGTGTTTAAAAG(+CAGTGT)GATCCACGAGGACAAGTTAGGTTGATGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605123250_2z07 GATACATACCATCAACCTAACTTGTCCTCGTG(+CAGTGT)GATCCTTTTAAACACTTCAACGCTCTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240605123248_u2v9 GTGAGTGACTTGGGGGTGGGTTTGAGTCTGGG(+CAGTGT)GTACCCAGTGACACAATTTTCTCATCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605123246_g30l TAGAGTTCCAGATGAGAAAATTGTGTCACTGG(+CAGTGT)GTACCCCAGACTCAAACCCACCCCCAAGTCA NGG 10 8 17 8 24 0 100 5 10