Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123244_v6v8 GAAACGTCCTTCCTCCCCTTCGCCCTGCCTGG(+CAGTGT)GATCCAGGTAGGGTGGTTGGACCTCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240605123242_o8dd ACAAGGATGGAGAGGTCCAACCACCCTACCTG(+CAGTGT)GATCCCAGGCAGGGCGAAGGGGAGGAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605123241_46et GAATGCCCTGGCATCTTGGTGGCATCTTGATG(+CAGTGT)GATCCAGCTCAGTCAGGACAGCTTTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605123239_g46l GCTCTGTGCTCAAAGCTGTCCTGACTGAGCTG(+CAGTGT)GATCCATCAAGATGCCACCAAGATGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605123238_8ydr CTCCTTGCAGGAGGGGATACCACCATTTCAGG(+CAGTGT)GATCCACAATTGAACCTCTGGCTTGATCCCT NGG 10 8 17 8 24 0 100 5 10
    240605123237_stxo CAGGGAGGGATCAAGCCAGAGGTTCAATTGTG(+CAGTGT)GATCCCTGAAATGGTGGTATCCCCTCCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605123234_f2sl GCCATCTACTGAGCGAGTGTGAACTGGCCAGG(+CAGTGT)GATCCACCAGGAAGAGGCATCATCTTCATTA NGG 10 8 17 8 24 0 100 5 10
    240605123233_7ohu CAATATAATGAAGATGATGCCTCTTCCTGGTG(+CAGTGT)GATCCCTGGCCAGTTCACACTCGCTCAGTAG NGG 10 8 17 8 24 0 100 5 10
    240605123232_woar ACCATGGAATGGATGCCCTGGTGTATTGTCTG(+CAGTGT)GATCCCCAGAAATACTTATCACCCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605123229_6k12 GTCCAGTGGCTGGGGTGATAAGTATTTCTGGG(+CAGTGT)GATCCAGACAATACACCAGGGCATCCATTCC NGG 10 8 17 8 24 0 100 5 10