Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605123228_9dwi CAGAGGCACATCCTTTTCCGTGGCAGCATCAG(+CAGTGT)GATCCACTCATGTTGCTGCTGGATGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605123227_1ipo GCTCTGCCCTCCATCCAGCAGCAACATGAGTG(+CAGTGT)GATCCTGATGCTGCCACGGAAAAGGATGTGC NGG 10 8 17 8 24 0 100 5 10
    240605123226_08a6 AACCTTGTGAGCAGCCAATCCAGTTCTAGCTG(+CAGTGT)GATCCCTCCACCCCACCCCCACCCCACCCCA NGG 10 8 17 8 24 0 100 5 10
    240605123224_pfbg TGGGGTGGGGTGGGGTGGGGGTGGGGTGGAGG(+CAGTGT)GATCCAGCTAGAACTGGATTGGCTGCTCACA NGG 10 8 17 8 24 0 100 5 10
    240605123223_655s TCTGTGTGAGTGTACACCACTTGTGTGGAGAG(+CAGTGT)GTACCCATGGAGCCCAGAGGTAGTGTATCCC NGG 10 8 17 8 24 0 100 5 10
    240605123220_87xq TCCAGGGGATACACTACCTCTGGGCTCCATGG(+CAGTGT)GTACCTCTCCACACAAGTGGTGTACACTCAC NGG 10 8 17 8 24 0 100 5 10
    240605123219_yqy2 TGCACACACCCATCTTCGCTACTCCAGACTAG(+CAGTGT)GATCCATAGCAATTGGGAAATGGGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240605123218_kp4a TACTCCCCAATGCCCATTTCCCAATTGCTATG(+CAGTGT)GATCCTAGTCTGGAGTAGCGAAGATGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605123216_t1xr CCCAGGAAATGCCTCCCTCCGGTCCAGGCAGG(+CAGTGT)GATCCCAGCACACACACCCCTGCTGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605123215_z0y2 CCCTCCACCACCAGCAGGGGTGTGTGTGCTGG(+CAGTGT)GATCCCTGCCTGGACCGGAGGGAGGCATTTC NGG 10 8 17 8 24 0 100 5 10