Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122645_acoe GTGGGCCTTTTAAATGTCCTATGGGATACAAG(+CAGTGT)GTACCAAGCCAGTGGAACCTGACAGAAATAT NGG 10 8 17 8 24 0 100 5 10
    240605122643_mmm0 TTGAAATATTTCTGTCAGGTTCCACTGGCTTG(+CAGTGT)GTACCTTGTATCCCATAGGACATTTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605122642_n496 TGCAGGAGGTTTGTCGCTGAAGCCATGCGATG(+CAGTGT)GATCCAGAGAGACTTTTTAATAATCCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605122641_6ra6 TATTACCTAAGGATTATTAAAAAGTCTCTCTG(+CAGTGT)GATCCATCGCATGGCTTCAGCGACAAACCTC NGG 10 8 17 8 24 0 100 5 10
    240605122638_sg09 TCCTGTGGTTTTGTAAACAGAGATATTGGGGG(+CAGTGT)GATCCTCCTTGAAATAGTTTGGAAGAATTGC NGG 10 8 17 8 24 0 100 5 10
    240605122636_ivb4 ACAGTGCAATTCTTCCAAACTATTTCAAGGAG(+CAGTGT)GATCCCCCCAATATCTCTGTTTACAAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605122634_dr79 AGAACTATACATAGTGAGACCATGTAACCCTG(+CAGTGT)GATCCTAACAACACAGTCTGTAAATAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605122633_oh2q AATAAACTTTTATTTACAGACTGTGTTGTTAG(+CAGTGT)GATCCAGGGTTACATGGTCTCACTATGTATA NGG 10 8 17 8 24 0 100 5 10
    240605122631_7ccm TAGGTGACATTCAGAGATGCTGTGTTGGGCTG(+CAGTGT)GTACCAGAATGCATTGATGTGTGTGCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605122630_8664 CAACGGTAAAGCACACACATCAATGCATTCTG(+CAGTGT)GTACCAGCCCAACACAGCATCTCTGAATGTC NGG 10 8 17 8 24 0 100 5 10