Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164107_kop2 CTTGGGGGACAGAGGACAGGGTGACTCTTGGG(+AGGACA)GTACCTCAATGCTTATGTATGTGGCCACAAG NGG 10 8 17 8 24 0 100 5 10
    240613164105_bccn TACGCCAGGCATGAGGGTCTGGCGTGTGCCTG(+AGGACA)GATCCTGTGCTCCTCCTTATAGGCACCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613164104_ni9z GAGTGGTGGGGTGCCTATAAGGAGGAGCACAG(+AGGACA)GATCCAGGCACACGCCAGACCCTCATGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613164103_15iy GAAAGGTGTGCACACTGGCATCACGGTCGACG(+AGGACA)GATCCACTGGGCATACATCAAGTGAGCGCAA NGG 10 8 17 8 24 0 100 5 10
    240613164101_p09j CCCAATTGCGCTCACTTGATGTATGCCCAGTG(+AGGACA)GATCCGTCGACCGTGATGCCAGTGTGCACAC NGG 10 8 17 8 24 0 100 5 10
    240613164059_o0f2 GAGTTCTGTTAACTCGTGGCTGATGTGTCCTG(+AGGACA)GATCCTAGGCACCGGTGGAGAAAGGTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240613164057_f4lm TATTTCTTACACCTTTCTCCACCGGTGCCTAG(+AGGACA)GATCCAGGACACATCAGCCACGAGTTAACAG NGG 10 8 17 8 24 0 100 5 10
    240613164055_xnnx AGAGCCACCTCGTGCATGGAGAGTGAGTCTGG(+AGGACA)GATCCAGTGAGAGTCACAATGGCAAGGACAT NGG 10 8 17 8 24 0 100 5 10
    240613164054_yhpe TATTGATGTCCTTGCCATTGTGACTCTCACTG(+AGGACA)GATCCCAGACTCACTCTCCATGCACGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613164053_nha7 TCAAGTTTATTAGACGAAAGCATAACCAAAAG(+AGGACA)GTACCTGAAGGGAACTGAGTTTTTCACAGTC NGG 10 8 17 8 24 0 100 5 10