Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164212_numt CGCCATGTCCCATTGGTGTAGTGGACAGCTGG(+AGGACA)GATCCCGGGTTTGAACGCACCATCTGCGCCA NGG 10 8 17 8 24 0 100 5 10
    240613164210_f5gi ACGTGGTTGGGGGCTTCTGGATCCTGGTGTGG(+AGGACA)GTACCAATCTCCCTTCATAGCTTTTCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613164209_f7h0 TAGGATCTGAGAAAAGCTATGAAGGGAGATTG(+AGGACA)GTACCCACACCAGGATCCAGAAGCCCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240613164207_hgl5 TCCTGGCCTCAGCACGTGGTTGGGGGCTTCTG(+AGGACA)GATCCTGGTGTGGGTACCAATCTCCCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613164205_sv9q AAGCTATGAAGGGAGATTGGTACCCACACCAG(+AGGACA)GATCCAGAAGCCCCCAACCACGTGCTGAGGC NGG 10 8 17 8 24 0 100 5 10
    240613164204_6p29 TGGATGAAGCATCTTTAACCTCATAATAAATG(+AGGACA)GATCCAGATTTTACCTCAAAGAATTATATTC NGG 10 8 17 8 24 0 100 5 10
    240613164203_98j9 AATAAGAATATAATTCTTTGAGGTAAAATCTG(+AGGACA)GATCCATTTATTATGAGGTTAAAGATGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613164202_mvvw ATCATAGAACAACTTGCACTTTCTGCTATATG(+AGGACA)GATCCAAGATATTGAACTGAGTGATCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613164200_d7os TTTCAAACCTGATCACTCAGTTCAATATCTTG(+AGGACA)GATCCATATAGCAGAAAGTGCAAGTTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613164159_peid TTTTCATCCTCCTGGGGATGTGGAAGCTGCAG(+AGGACA)GATCCCTGAGATGAAGTGCAGTGTGGAGCCT NGG 10 8 17 8 24 0 100 5 10