Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164248_f81i TAAATGTCTTTTTTTTTCTAAGTACAAAGGAG(+AGGACA)GTACCTTCGCATAAAACATGAAGGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240613164246_k96b ATGACTGATATCTATAGCCCAGGAACCGCCTG(+AGGACA)GATCCAGGATTGTGAAACTAAGCCCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613164244_9f53 GTCCCTCCCACGGGCTTAGTTTCACAATCCTG(+AGGACA)GATCCAGGCGGTTCCTGGGCTATAGATATCA NGG 10 8 17 8 24 0 100 5 10
    240613164243_x9ca TCCTCTACAAGGATACTGTGGCTAATGCACAG(+AGGACA)GTACCCTGATTGCAGAGAAAACTCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613164242_4zdv TATCTATGCCCAGAGTTTTCTCTGCAATCAGG(+AGGACA)GTACCTGTGCATTAGCCACAGTATCCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240613164240_gbbu AAGGTACTACCGGCTATAGCCACAGGGATCCG(+AGGACA)GTACCATTGAGATCCCTTCATAAAATATGGA NGG 10 8 17 8 24 0 100 5 10
    240613164239_3mpy CTTGCTCCATATTTTATGAAGGGATCTCAATG(+AGGACA)GTACCGGATCCCTGTGGCTATAGCCGGTAGT NGG 10 8 17 8 24 0 100 5 10
    240613164236_7pn2 TGCTCCCGCCTATCTTGCTCCTTAACACTGGG(+AGGACA)GATCCGATAGAGAAATGACACAAGGTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613164235_n3a3 ACACACTGAAACCTTGTGTCATTTCTCTATCG(+AGGACA)GATCCCCAGTGTTAAGGAGCAAGATAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240613164234_ozu4 GCTCCTAAGGTACTACCGGCTATAGCCACAGG(+AGGACA)GATCCGGTACCATTGAGATCCCTTCATAAAA NGG 10 8 17 8 24 0 100 5 10